FN Archimer Export Format
PT J
TI Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin
BT 
AF GUEGUEN, Yannick
   ROMESTAND, Bernard
   FIEVET, Julie
   SCHMITT, Paulina
   DESTOUMIEUX GARZON, Delphine
   FRANCK, Vandenbulcke
   PHILIPPE, Bulet
   BACHERE, Evelyne
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:1;
FF 1:PDG-DOP-DCM-BOME-LALR;2:;3:PDG-DOP-DCM-BOME-LALR;4:PDG-DOP-DCM-BOME-LALR;5:;6:;7:;8:PDG-DOP-DCM-BOME-LALR;
C1 Univ Montpellier 2, CNRS, Ifremer, UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France.
   Univ Lille 1, EA 3570, Lab Ecol Numer & Ecotoxicol, F-59655 Villeneuve Dascq, France.
   UJF, CNRS, TIMC IMAG, UMR 5525, F-74160 Archamps, France.
C2 UNIV MONTPELLIER, FRANCE
   UNIV LILLE, FRANCE
   UNIV GRENOBLE, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
   PALAVAS
   PALAVAS,MONTPELLIER
SE PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.202
TC 66
UR https://archimer.ifremer.fr/doc/2009/publication-6232.pdf
LA English
DT Article
DE ;Antimicrobial peptide;Mollusk;Synergy;Invertebrate;Innate immunity;Pacific oyster
AB A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an invertebrate. By in situ hybridization, the expression of Cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. Cg-prp transcripts were also detected in hemocytes infiltrating mantle, where Cg-Def is expressed. Additionally, by immunocytochemistry, we showed that Cg-Prp or one of its variants is present in some hemocytes together with defensins. In conclusion, we described here the first proline-rich AMP from mollusk. From our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins. (C) 2008 Elsevier Ltd. All rights reserved.
PY 2009
PD FEB
SO Molecular Immunology
SN 0161-5890
PU Elsevier
VL 46
IS 4
UT 000263429500002
BP 516
EP 522
DI 10.1016/j.molimm.2008.07.021
ID 6232
ER

EF