FN Archimer Export Format
PT J
TI Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid
BT 
AF LITAKER, R. Wayne
   STEWART, Thomas N.
   EBERHART, Bich-thuy L.
   WEKELL, John C.
   TRAINER, Vera L.
   KUDELA, Raphael M.
   MILLER, Peter E.
   ROBERTS, Alice
   HERTZ, Cassandra
   JOHNSON, Tyler A.
   FRANKFURTER, Greg
   SMITH, G. Jason
   SCHNETZER, Astrid
   SCHUMACKER, Joe
   BASTIAN, Jonnette L.
   ODELL, Anthony
   GENTIEN, Patrick
   LE GAL, Dominique
   HARDISON, I. Ransom
   TESTER, Patricia A.
AS 1:1;2:2;3:3;4:3;5:3;6:4;7:4;8:4;9:4;10:4;11:5;12:5;13:6;14:7;15:7;16:8;17:9;18:10;19:1;20:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:PDG-DOP-DCB-DYNECO-PELAGOS;18:PDG-DOP-LER-LERFBN;19:;20:;
C1 NOAA, Natl Ocean Serv, Beaufort, NC 28516 USA.
   Mercury Sci Inc, Raleigh, NC 27607 USA.
   NOAA, NW Fisheries Sci Ctr, Seattle, WA 98112 USA.
   Univ Calif Santa Cruz, Inst Marine Sci, Santa Cruz, CA 95064 USA.
   Moss Landing Marine Labs, Moss Landing, CA 95039 USA.
   Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA.
   Quinault Indian Nation, Div Nat Resources, Dept Fisheries, Taholah, WA 98587 USA.
   Olymp Reg Harmful Algal Bloom Partnership, Hoquiam, WA 98550 USA.
   IFREMER, Ctr Brest, DYNECO, F-29280 Plouzane, France.
   IFREMER, F-29900 Concarneau, France.
C2 NOAA, USA
   MERCURY SCI INC, USA
   NOAA, USA
   UNIV CALIF SANTA CRUZ, USA
   MOSS LANDING MARINE LABS, USA
   UNIV SO CALIF, USA
   QUINAULT INDIAN NATION, USA
   OLYMP REG HARMFUL ALGAL BLOOM PARTNERSHIP, USA
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
   CONCARNEAU
SE PDG-DOP-DCB-DYNECO-PELAGOS
   PDG-DOP-LER-LERFBN
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 0.576
TC 28
UR https://archimer.ifremer.fr/doc/2008/publication-6253.pdf
LA English
DT Article
DE ;Test kit;Razor clams;Scallops;Mussels;ELISA;Domoic acid poisoning;ASP
AB Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting. diarrhea, and in more severe cases confusion. loss of memory. disorientation. and even coma or death. This paper describes the development and validation of a rapid. sensitive. enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography. fluorenylmetbox-yearbonyl high performance liquid chromatography, or liquid chromatography-mass spectrometry methods. It has a linear range from 1.1-3 ppb and was used successfully to measure DA in razor clams, mussels, scallops, and phytoplankton. The assay requires approximately 1.5 h to complete and has a standard 96-well format where each strip of eight wells is removable and can be stored at 4 degrees C until needed. The first two wells of each strip serve as an internal control climinating the need to run a standard curve. This allows as few as 3 or as many as 36 duplicate samples to be run at a time enabling real-time sample processing and limiting degradation of DA. which can occur during storage. There was minimal cross-reactivity in this assay with glutamine, glutamic acid. kainic acid. epi- or iso-DA. This accurate. rapid. cost-effective. assay offers environmental managers and public health officials an effective tool for monitoring DA concentrations in environment samples.
PY 2008
PD DEC
SO Journal of Shellfish Research
SN 0730-8000
PU National Shellfisheries Association
VL 27
IS 5
UT 000261712200030
BP 1301
EP 1310
ID 6253
ER

EF