FN Archimer Export Format
PT C
TI Molecular characterization of parasites of the genus Perkinsus present in clams from French producing areas
BT 
AF ARZUL, Isabelle
   MICHEL, Justine
   CHOLLET, Bruno
   ROBERT, Maeva
   MIOSSEC, Laurence
   GARCIA, Celine
   FRANCOIS, Cyrille
AS 1:;2:;3:;4:;5:;6:;7:;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCB-DYNECO-VIGIES;6:PDG-DOP-DCN-AGSAE-LGP;7:PDG-DOP-DCN-AGSAE-LGP;
SI LA TREMBLADE
   NANTES
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCB-DYNECO-VIGIES
UR https://archimer.ifremer.fr/doc/2009/acte-6269.pdf
LA English
DT Proceedings paper
DE ;French producing areas;Clams;Perkinsus olseni;Parasite
AB Production of clams is the third most important bivalve production in France and was estimated at 3400 metric tons in 2001 among which 60% came from aquaculture and 40% from fisheries. Surveillance of clam diseases mainly relies on histology and thioglycolate culture, two techniques allowing detection of parasites of the genus Perkinsus but not specific enough to determine the parasite species. Based on a previous study aiming at estimating distribution and prevalence of perkinsosis in France, we selected 34 infected clams from 4 different producing areas in order to characterize the parasite species. Molecular characterization was done by PCR-RFLP targeting the ITS region as described by Abollo et al. (2006). 16 PCR products were cloned and 10 to 20 clones per individual were selected for PCR-RFLP to test potential intra individual polymorphism. 23 clones, including the ones which yielded non expected restriction profiles, were finally selected for sequencing. All the obtained sequences displayed 99 to 100% of homology with Perkinsus olseni confirming the presence of this protozoan in at least four different French marine areas (Golfe du Morbihan, Thau, Leucate, Arcachon). Polymorphism between clones obtained from a same clam or from different clams did not appear geographic dependent.
PY 2009
PD MAR
CT Presentation 101st Annual Meeting National Shellfisheries Association (NSA), Savannah, Georgia, March 22-26, 2009
ID 6269
ER

EF