||Morga Benjamin1, Arzul Isabelle1, Chollet Bruno1, Renault Tristan1
||1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
||Fish & Shellfish Immunology (1050-4648) (Elsevier), 2009-06 , Vol. 26 , N. 6 , P. 836-842
|WOS© Times Cited
||Reactive oxygen species (ROS), Esterase, Flow cytometry, Haemocytes, Ostrea edulis, Bonamia ostreae
||Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities and ROS production by haemocytes after incubation with live parasites, while there was little difference in phagocytosis activity when compared with controls. Similarly, dead parasites induced a decrease in haemocyte activities but to a lesser extent compared to live parasites. These results suggest that B. ostreae actively contributes to the modification of haemocyte activities in order to ensure its own intracellular survival.