FN Archimer Export Format PT J TI Production of exopolysaccharides by Antarctic marine bacterial isolates BT AF NICHOLS, C GARON, Sandrine BOWMAN, J RAGUENES, Gerard GUEZENNEC, Jean AS 1:;2:;3:;4:;5:; FF 1:;2:PDG-DRV-VP-BMM;3:;4:PDG-DRV-VP-BMM;5:PDG-DRV-VP-BMM; C1 Univ Tasmania, Sch Agr Sci, Hobart, Tas 7000, Australia. Inst Francais Rech Exploitat Mer, Ctr Brest, F-29280 Plouzane, France. C2 UNIV TASMANIA, AUSTRALIA IFREMER, FRANCE SI BREST SE PDG-DRV-VP-BMM IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 1.835 TC 146 UR https://archimer.ifremer.fr/doc/2004/publication-629.pdf LA English DT Article DE ;Sea ice;Pseudoalteromonas spp.;Particulate organic material;Exopolysaccharides;Antarctic marine bacteria AB Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10degreesC than at 20degreesC. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. Conclusions: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. Significance and Impact of the Study: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications. PY 2004 PD MAY SO Journal of Applied Microbiology SN 1364-5072 PU Blackwell science VL 96 IS 5 UT 000221329000018 BP 1057 EP 1066 DI 10.1111/j.1365-2672.2004.02216.x ID 629 ER EF