A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments

Type Article
Date 2005-08
Language English
Author(s) McCombie Helen1, Ledu Christophe1, Phelipot Pascal1, Lapegue Sylvie1, Boudry PierreORCID1, Gerard Andre2
Affiliation(s) 1 : Inst Francais Rech Exploitat Mer, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : IFREMER, Ctr Nantes, F-44311 Nantes, France.
Source Marine Biotechnology (1436-2228) (Springer), 2005-08 , Vol. 7 , N. 4 , P. 318-330
DOI 10.1007/s10126-004-0440-2
WOS© Times Cited 15
Keyword(s) Oyster breeding, Gynogenesis, Polar body, Cytochalasin B, Polyploidy, Crassostrea gigas
Abstract We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring.
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McCombie Helen, Ledu Christophe, Phelipot Pascal, Lapegue Sylvie, Boudry Pierre, Gerard Andre (2005). A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments. Marine Biotechnology, 7(4), 318-330. Publisher's official version : https://doi.org/10.1007/s10126-004-0440-2 , Open Access version : https://archimer.ifremer.fr/doc/00000/632/