FN Archimer Export Format
PT J
TI A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
BT 
AF MCCOMBIE, Helen
   LEDU, Christophe
   PHELIPOT, Pascal
   LAPEGUE, Sylvie
   BOUDRY, Pierre
   GERARD, Andre
AS 1:1;2:1;3:1;4:1;5:1;6:2;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DPS;
C1 Inst Francais Rech Exploitat Mer, Lab Genet & Pathol, F-17390 La Tremblade, France.
   IFREMER, Ctr Nantes, F-44311 Nantes, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DPS
IN WOS Ifremer jusqu'en 2018
IF 1.545
TC 17
UR https://archimer.ifremer.fr/doc/2005/publication-632.pdf
LA English
DT Article
DE ;Oyster breeding;Gynogenesis;Polar body;Cytochalasin B;Polyploidy;Crassostrea gigas
AB We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring.
PY 2005
PD AUG
SO Marine Biotechnology
SN 1436-2228
PU Springer
VL 7
IS 4
UT 000231503200007
BP 318
EP 330
DI 10.1007/s10126-004-0440-2
ID 632
ER

EF