AMP-deaminase in elasmobranch fish: A comparative histochemical and enzymatic study

Type Article
Date 2005-08
Language English
Author(s) Thebault Marie-T1, Izem Lahoucine2, Leroy Jean Paul3, Gobin Eric3, Charrier Gregory1, Raffin Jean-Paul5
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR, CNRS UMR 6539, F-29280 Plouzane, France.
2 : Museum Natl Hist Nat, Stn Biol Marine, Concarneau, France.
3 : Coll France, Concarneau, France.
4 : CHU Morvan, Lab Anat Pathol, F-29609 Brest, France.
5 : IFREMER, Lab Mirobiol Millieux Extremes, UMR 6197, F-29280 Plouzane, France.
Source Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology (1096-4959) (Elsevier), 2005-08 , Vol. 141 , N. 4 , P. 472-479
DOI 10.1016/j.cbpc.2005.05.009
WOS© Times Cited 4
Keyword(s) White fibres, Red fibres, Muscle, Histology, Fish, Evolution, Elasmobranch, AMP deaminase
Abstract AMP-deaminase activity was measured in white muscle from a wide range of fish, including one cyclostome, 13 chondrosteans, and one teleost to elucidate the pattern of the AMP-deaminase activity in white muscle of fish. Compared to a mammalian (rat) muscle extract, low enzyme activities are found in the cyclostome and two elasmobranchs from two families (Scyliorhinidae, Hexanchidae). In contrast, higher AMP-deaminase activities, similar to mammals, are expressed in Squalidae, all families of skates, Chimaeridae and in the teleostean fish. We then compared AMP-deaminase activities in red and white muscles from two representative elasmobranch fish, the dogfish (Scyliorhinus canicula) and the thornback ray (Raja clavata). The fibre type composition and distribution of the locomotory musculature were determined in these two elasmobranchs to establish a relationship between the morphology, the type of fibres of the locomotion-implicated muscles and the AMP-deaminase activity. Experimental data are discussed with respect to the layout of fibres in the myotome. In both species, three fibre types were identified. In the two fish myotomes, most of the axial muscles are white fibres while red fibres constitute a thin sheet. Some differences were observed between the two species in the distribution of intermediate fibres: in dogfish, these are located between the red and white fibres; in thornback ray, some are dispersed within the white fibre region, while others form an intermediary layer like in dogfish. These results suggest that in the course of evolution, an amplification of the AMP-deaminase activity in muscle was coupled with increase of complexity of the muscular structure.
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Thebault Marie-T, Izem Lahoucine, Leroy Jean Paul, Gobin Eric, Charrier Gregory, Raffin Jean-Paul (2005). AMP-deaminase in elasmobranch fish: A comparative histochemical and enzymatic study. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 141(4), 472-479. Publisher's official version : https://doi.org/10.1016/j.cbpc.2005.05.009 , Open Access version : https://archimer.ifremer.fr/doc/00000/635/