FN Archimer Export Format
PT J
TI Enzymatic hydrolysis of cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) viscera using commercial proteases: Effects on lipid distribution and amino acid composition
BT 
AF KECHAOU, Emna
   DURNAY, Justine
   DONNAY-MORENO, Claire
   JAOUEN, Pascal
   GOUYGOU, Jean-Paul
   BERGE, Jean-Pascal
   BEN ARNAR, Raja
AS 1:2,3;2:1;3:1;4:2;5:1;6:1;7:3;
FF 1:PDG-DOP-DCN-STAM;2:;3:PDG-DOP-DCN-STAM;4:;5:PDG-DOP-DCN-STAM;6:PDG-DOP-DCN-STAM;7:;
C1 IFREMER, Dept STAM, F-44311 Nantes, France.
   Univ Nantes, GEPEA, CNRS, UMR 6144, F-44602 St Nazaire, France.
   Fac Sci Sfax, Lab Sci Mat & Environm, Sfax 3018, Tunisia.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   FAC SCI SFAX, TUNISIA
SI NANTES
SE PDG-DOP-DCN-STAM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 1.749
TC 55
UR https://archimer.ifremer.fr/doc/2009/publication-6366.pdf
LA English
DT Article
DE ;Sardine;Viscera;Proteolysis;Marine lipids;Cuttlefish;By products
AB Total lipid and phospholipid recovery as well as amino acid quality and composition from cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) were compared. Enzymatic hydrolyses were performed using the three proteases Protamex, Alcalase, and Flavourzyme by the pH-stat method (24 h, pH 8, 50 degrees C). Three fractions were generated: an insoluble sludge, a soluble aqueous phase, and an oily phase. For each fraction, lipids, phospholipids, and proteins were quantified. Quantitative and qualitative analyses of the raw material and hydrolysates were performed. The degree of hydrolysis (DH) for cuttlefish viscera was 3.2% using Protamex, 6.8% using Flavourzyme, and 7% using Alcalase. DH for sardine viscera was 1.9% (using Flavourzyme), 3.1% (using Protamex) and 33% (using Alcalase). Dry matter yields of all hydrolysis reactions increased in the aqueous phases. Protein recovery following hydrolysis ranged from 57.2% to 64.3% for cuttlefish and 57.4% to 61.2% for sardine. Tissue disruption following protease treatment increased lipid extractability, leading to higher total lipid content after hydrolysis. At least 80% of the lipids quantified in the raw material were distributed in the liquid phases for both substrates. The hydrolysed lipids were richer in phospholipids than in the lipids extracted by classical chemical extraction, especially after Flavourzyme hydrolysis for cuttlefish and Alcalase hydrolysis for sardine. The total amino acid content differed according to the substrate and the enzyme used. However, regardless of the raw material or the protease used, hydrolysis increased the level of essential amino acids in the hydrolysates, thereby increasing their potential nutritional value for feed products. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
PY 2009
PD FEB
SO Journal of Bioscience and Bioengineering
SN 1389-1723
PU Elsevier
VL 107
IS 2
UT 000264671200012
BP 158
EP 164
DI 10.1016/j.jbiosc.2008.10.018
ID 6366
ER

EF