FN Archimer Export Format PT J TI Structural and functional characterizations of an Activin type II receptor orthologue from the pacific oyster Crassostrea gigas BT AF LE QUERE, Herve HERPIN, Amaury HUVET, Arnaud LELONG, Christophe FAVREL, Pascal AS 1:1;2:3;3:2;4:1;5:1; FF 1:;2:;3:PDG-DOP-DCB-PFOM-PI;4:;5:; C1 Univ Caen Basse Normandie Physiol & Ecophysiol Mo, UMR M100, IFREMER, IBFA,IFR ICORE 146, F-14032 Caen, France. Univ Caen Basse Normandie Physiol & Ecophysiol Mo, UMR M100, Ctr IFREMER, F-29280 Plouzane, France. Univ Wurzburg, Dept Physiol Chem 1, D-97074 Wurzburg, Germany. C2 UNIV CAEN, FRANCE UNIV CAEN, FRANCE UNIV WURZBURG, GERMANY IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 2.416 TC 16 UR https://archimer.ifremer.fr/doc/2009/publication-6401.pdf LA English DT Article DE ;Activin receptor;Nervous tissues;Development;Lophotrochozoa;Mollusc AB Members of the Transforming Growth factor beta (TGF-beta) superfamily of cell signalling polypeptides are known to play important roles in cell proliferation and differentiation during development and in various physiological processes of most animal clades. Recent findings in the mollusc Crassostrea gigas demonstrate the occurrence of a diversity of TGF-beta signalling components including various ligands, three type I receptors but only a single type II receptor. This report describes the characterization of Cg-ActRII, a new type II receptor displaying homology with vertebrate and Drosophila Activin type II receptors. The use of zebrafish embryo as a reporter organism revealed that, in a way similar to its zebrafish counterpart, overexpression of Cg-ActRII or its dominant negative acting truncated form resulted in a dose dependent range of dorsoventral defects coupled with anterior disorders. Expression pattern of Cg-ActRII transcripts examined by real time PCR and in situ PCR in C gigas showed high levels of Cg-ActRII transcripts in early embryonic stages and in the developing larval central nervous system. Except for a high expression in the visceral ganglia, most oyster adult tissues displayed rather low levels of transcripts. Altogether, the data suggest a high degree of conservation at both the structural and functional levels during evolution for this class of receptors. (C) 2009 Elsevier B.V. All rights reserved. PY 2009 PD MAY SO Gene SN 0378-1119 PU Elsevier VL 436 IS 1-2 UT 000265343000013 BP 101 EP 107 DI 10.1016/j.gene.2009.01.010 ID 6401 ER EF