Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

Type Article
Date 2009-05
Language English
Author(s) Sauvage Christopher1, Boudry PierreORCID1, Lapegue Sylvie1
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol, La Tremblade, France.
Source Molecular Ecology Resources (1755-098X) (Wiley / Blackwell), 2009-05 , Vol. 9 , N. 3 , P. 853-855
DOI 10.1111/j.1755-0998.2009.02525.x
WOS© Times Cited 23
Keyword(s) Pacific oyster, Null alleles, EST SSR markers, Crassostrea gigas
Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
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