FN Archimer Export Format PT J TI Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp BT AF LABREUCHE, Yannick O'LEARY, Nuala A. DE LA VEGA, Enrique VELOSO, Artur GROSS, Paul S. CHAPMAN, Robert W. BROWDY, Craig L. WARR, Gregory W. AS 1:1,4;2:1;3:1;4:1;5:2;6:3;7:3;8:1,3; FF 1:PDG-DOP-DCOP-LEADNC-LAC;2:;3:;4:;5:;6:;7:;8:; C1 Med Univ S Carolina, Marine Biomed & Environm Sci Ctr, Charleston, SC 29412 USA. Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA. Marine Resources Res Inst, Dept Nat Resources, Charleston, SC 29412 USA. IFREMER, Département Lagon, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie, BP 2059, 98846 Nouméa Cedex, New Caledonia C2 UNIV MED S CAROLINA, USA UNIV MED S CAROLINA, USA MRRI, USA IFREMER, FRANCE SI SAINT VINCENT SE PDG-DOP-DCOP-LEADNC-LAC IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 3.29 TC 36 UR https://archimer.ifremer.fr/doc/2009/publication-6428.pdf LA English DT Article DE ;Shrimp;Virus;RNA interference;Invertebrate immunity;Innate immune response;Toll like receptor;DSRNA AB injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection from white spot syndrome virus and did not increase the shrimp susceptibility to viral infection, when compared to controls. In contrast, gene-specific dsRNA injected to specifically silence lToll expression activated an antiviral response. These data strongly suggest that shrimp lToll plays no role in dsRNA-induced antiviral immunity. (C) 2009 Elsevier Ltd. All rights reserved. PY 2009 PD JUN SO Developmental & Comparative Immunology SN 0145-305X PU Elsevier VL 33 IS 7 UT 000266152600002 BP 806 EP 810 DI 10.1016/j.dci.2009.02.005 ID 6428 ER EF