FN Archimer Export Format
PT J
TI Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
BT 
AF ARZUL, Isabelle
   GAGNAIRE, Beatrice
   BOND, Celine
   CHOLLET, Bruno
   MORGA, Benjamin
   FERRAND, Sylvie
   ROBERT, Maeva
   RENAULT, Tristan
AS 1:1;2:2;3:1;4:1;5:1;6:1;7:1;8:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP;7:PDG-DOP-DCN-AGSAE-LGP;8:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, LGP, F-17390 La Tremblade, France.
   IRSN, Lab Radioecol & Ecotoxicol, DEI SECRE LRE, F-13115 St Paul Les Durance, France.
C2 IFREMER, FRANCE
   IRSN, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 1.687
TC 43
UR https://archimer.ifremer.fr/doc/2009/publication-6443.pdf
LA English
DT Article
DE ;Salinity tolerance;Temperature tolerance;Cell viability;In vitro assays;Flow cytometry;Bonamia ostreae
AB Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of incubation for flow cytometry analyses including estimation of parasite mortality and parasite viability through detection of non-specific esterase activities. Artificial seawater appeared unsuitable for parasite survival, and results for all media showed a significantly lower survival at 25°C compared to 4°C and 15°C. Moreover, high salinities (≥35 g l–1) favoured parasite survival and detection of esterase activities. Flow cytometry appears to be a suitable technique to investigate survival and activities of unicellular parasites like B. ostreae under varied conditions. Although these results contribute to a better understanding of existing interactions between the parasite B. ostreae and its environment, validation through epidemiological surveys in the field is also needed.
PY 2009
PD MAY
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 85
IS 1
UT 000267102600010
BP 67
EP 75
DI 10.3354/dao02047
ID 6443
ER

EF