FN Archimer Export Format
PT J
TI The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas
BT 
AF FABIOUX, Caroline
   POUVREAU, Stephane
   LEROUX, Frederique
   HUVET, Arnaud
AS 1:;2:;3:;4:;
FF 1:PDG-DRV-RA-PI;2:PDG-DRV-RA-PI;3:PDG-DRV-RA-LGP;4:PDG-DRV-RA-PI;
C1 IFREMER, Ctr Brest, UMR Physiol & Ecophysiol Mollusques Marins, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, CNRS, Lab Sci Environm Marin, IUEM,UMR 6539, F-29280 Plouzane, France.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
   UNIV BRETAGNE OCCIDENTALE, FRANCE
   IFREMER, FRANCE
SI BREST
   ARGENTON
   LA TREMBLADE RONCE
SE PDG-DRV-RA-PI
   PDG-DRV-RA-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 2.904
TC 77
UR https://archimer.ifremer.fr/doc/2004/publication-646.pdf
LA English
DT Article
DE ;RNA helicase;DEAD box;Vasa;Germline;Gametogenesis;Oyster
AB The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation proceeded. These data and the evolutionary conservation observed suggested the role of Oyvlg in germline development. Oyvlg is the first germ cell specific marker in oyster and will be very useful in studies of oyster germline formation. 
PY 2004
PD MAR
SO Biochemical and Biophysical Research Communications
SN 0006-291X
PU Elsevier
VL 315
IS 4
UT 000220105600019
BP 897
EP 904
DI 10.1016/j.bbrc.2004.01.145
ID 646
ER

EF