Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas

Type Article
Date 2004-07
Language English
Author(s) Fabioux Caroline, Huvet ArnaudORCID, Lelong C, Robert ReneORCID, Pouvreau StephaneORCID, Daniel Jean-Yves, Mingant Christian, Le Pennec M
Affiliation(s) IFREMER, UMR Physiol & Ecophysiol Mollusques Marins, F-29280 Plouzane, France.
Univ Bretagne Occidentale, UMR CNRS 6539, IUEM, Lab Sci Environm Marin, F-29280 Plouzane, France.
Univ Caen, UMR Physiol & Ecophysiol Mollusques Marins, F-14032 Caen, France.
Source Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-07 , Vol. 320 , N. 2 , P. 592-598
DOI 10.1016/j.bbrc.2004.06.009
WOS© Times Cited 105
Keyword(s) Primordial germ cells, Oyster vasa like gene, Germline, Development, Crassostrea gigas
Abstract The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae body and that would correspond to primordial germ cells (PGCs). Based on these results, we postulate that the germline of C. gigas is specified at early development by maternal cytoplasmic determinants including Oyvlg mRNAs, in putative PGCs that would differentiate into germinal stem cells in juvenile oysters.
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Fabioux Caroline, Huvet Arnaud, Lelong C, Robert Rene, Pouvreau Stephane, Daniel Jean-Yves, Mingant Christian, Le Pennec M (2004). Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas. Biochemical and Biophysical Research Communications, 320(2), 592-598. Publisher's official version : https://doi.org/10.1016/j.bbrc.2004.06.009 , Open Access version : https://archimer.ifremer.fr/doc/00000/648/