FN Archimer Export Format
PT J
TI Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas
BT 
AF FABIOUX, Caroline
   HUVET, Arnaud
   LELONG, C
   ROBERT, Rene
   POUVREAU, Stephane
   DANIEL, Jean-Yves
   MINGANT, Christian
   LE PENNEC, M
AS 1:;2:;3:;4:;5:;6:;7:;8:;
FF 1:PDG-DRV-RA-PI;2:PDG-DRV-RA-PI;3:;4:PDG-DRV-RA-PI;5:PDG-DRV-RA-PI;6:PDG-DRV-RA-PI;7:PDG-DRV-RA-PI;8:;
C1 IFREMER, UMR Physiol & Ecophysiol Mollusques Marins, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, UMR CNRS 6539, IUEM, Lab Sci Environm Marin, F-29280 Plouzane, France.
   Univ Caen, UMR Physiol & Ecophysiol Mollusques Marins, F-14032 Caen, France.
C2 IFREMER, FRANCE
   UNIV BRETAGNE OCCIDENTALE, FRANCE
   UNIV CAEN, FRANCE
SI BREST
   ARGENTON
SE PDG-DRV-RA-PI
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 2.904
TC 105
UR https://archimer.ifremer.fr/doc/2004/publication-648.pdf
LA English
DT Article
DE ;Primordial germ cells;Oyster vasa like gene;Germline;Development;Crassostrea gigas
AB The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae body and that would correspond to primordial germ cells (PGCs). Based on these results, we postulate that the germline of C. gigas is specified at early development by maternal cytoplasmic determinants including Oyvlg mRNAs, in putative PGCs that would differentiate into germinal stem cells in juvenile oysters. 
PY 2004
PD JUN
SO Biochemical and Biophysical Research Communications
SN 0006-291X
PU Elsevier
VL 320
IS 2
UT 000222567700047
BP 592
EP 598
DI 10.1016/j.bbrc.2004.06.009
ID 648
ER

EF