FN Archimer Export Format
PT J
TI Estimation of Pig Fecal Contamination in a River Catchment by Real-Time PCR Using Two Pig-Specific Bacteroidales 16S rRNA Genetic Markers
BT 
AF MIESZKIN, Sophie
   FURET, Jean-Pierre
   CORTHIER, Gerard
   GOURMELON, Michele
AS 1:1;2:2;3:2;4:1;
FF 1:PDG-DOP-DCN-EMP-MIC;2:;3:;4:PDG-DOP-DCN-EMP-MIC;
C1 IFREMER, Microbiol Lab, EMP, F-29280 Plouzane, France.
   INRA, UEPSD, F-78352 Jouy En Josas, France.
C2 IFREMER, FRANCE
   INRA, FRANCE
SI BREST
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
IF 3.686
TC 121
UR https://archimer.ifremer.fr/doc/2009/publication-6493.pdf
LA English
DT Article
AB The microbiological quality of coastal or river water can be affected by fecal contamination from human or animal sources. To discriminate pig fecal pollution from other pollution, a library-independent microbial source tracking method targeting Bacteroidales host-specific 16S rRNA gene markers by real-time PCR was designed. Two pig-specific Bacteroidales markers (Pig-1-Bac and Pig-2-Bac) were designed using 16S rRNA gene Bacteroidales clone libraries from pig feces and slurry. For these two pig markers, 98 to 100% sensitivity and 100% specificity were obtained when tested by TaqMan real-time PCR. A decrease in the concentrations of Pig-1-Bac and Pig-2-Bac markers was observed throughout the slurry treatment chain. The two newly designed pig-specific Bacteroidales markers, plus the human-specific (HF183) and ruminant-specific (BacR) Bacteroidales markers, were then applied to river water samples (n = 24) representing 14 different sites from the French Daoulas River catchment (Brittany, France). Pig-1-Bac and Pig-2-Bac were quantified in 25% and 62.5%, respectively, of samples collected around pig farms, with concentrations ranging from 3.6 to 4.1 log10 copies per 100 ml of water. They were detected in water samples collected downstream from pig farms but never detected near cattle farms. HF183 was quantified in 90% of water samples collected downstream near Daoulas town, with concentrations ranging between 3.6 and 4.4 log10 copies per 100 ml of water, and BacR in all water samples collected around cattle farms, with concentrations ranging between 4.6 and 6.0 log10 copies per 100 ml of water. The results of this study highlight that pig fecal contamination was not as frequent as human or bovine fecal contamination and that fecal pollution generally came from multiple origins. The two pig-specific Bacteroidales markers can be applied to environmental water samples to detect pig fecal pollution.
PY 2009
PD MAY
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 75
IS 10
UT 000265908500007
BP 3045
EP 3054
DI 10.1128/AEM.02343-08
ID 6493
ER

EF