FN Archimer Export Format
PT J
TI The identification of genes from the oyster Crassostrea gigas that are differentially expressed in progeny exhibiting opposed susceptibility to summer mortality
BT 
AF HUVET, Arnaud
   HERPIN, Amaury
   DEGREMONT, Lionel
   LABREUCHE, Yannick
   SAMAIN, Jean-Francois
   CUNNINGHAM, Charles
AS 1:;2:;3:;4:;5:;6:;
FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:PDG-DRV-RA-LGP;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-PI;6:;
C1 IFREMER, Ctr Brest, Unite Mixte Rech Physiol & Ecophysiol Mollusques, F-29280 Plouzane, France.
   Ctr High Technol, Sars Int Ctr Mol Marine Biol, N-5008 Bergen, Norway.
   Lab Ifremer Genet Pathol, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
   CTR HIGH TECHNOL, NORWAY
   LAB IFREMER, FRANCE
SI BREST
   LA TREMBLADE
SE PDG-DOP-DCB-PFOM-PI
   PDG-DRV-RA-LGP
IN WOS Ifremer jusqu'en 2018
IF 2.705
TC 119
UR https://archimer.ifremer.fr/doc/2004/publication-651.pdf
LA English
DT Article
DE ;Suppression subtractive hybridization;Oysters;Differentially regulated genes;Bivalves;Bacterial challenge
AB Summer mortality associated with juveniles of the oyster Crassostrea gigas is probably the result of a complex interaction between the host, pathogens and environmental factors. Genetic variability in the host appears to be a major determinant in its sensitivity to summer mortality. Previously, divergent selection criteria based on summer survival have been applied to produce oyster families with resistant and susceptible progeny. In this paper, we describe the use of suppression subtractive hybridization to generate 150 C gigas clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these clones was determined. In 28%, the inferred amino sequence was found to match the products of known genes, 14% matched hypothetical proteins and a further 14% appeared to contain open reading frames (ORFs) whose product had no obvious homologue in the nucleotide databases. It has been hypothesized that differences exist in the level of energy generation and immune function between resistant and susceptible progeny. In light of this, clones encoding homologues of cavortin, cyclophilin, isocitrate dehydrogenase, sodium glucose cotransporter, fatty acid binding protein, ATPase H+ transporting lysosomal protein, precerebellin, and scavenger receptor were analyzed by real-time PCR. These transcripts were induced in resistant progeny when compared to their susceptible counterparts. A bacterial challenge of oysters resulted in the suppression of six of these transcripts in only those that were resistant to summer mortality. This study has identified potential candidates for further investigation into the functional basis of resistance and susceptibility to summer mortality. 
PY 2004
PD DEC
SO Gene
SN 0378-1119
PU Elsevier
VL 343
IS 1
UT 000225728700021
BP 211
EP 220
DI 10.1016/j.gene.2004.09.008
ID 651
ER

EF