FN Archimer Export Format
PT J
TI In vitro study on sperm competition in common carp (Cyprinus carpio L.)
BT 
AF KASPAR, Vojtech
   HULAK, Martin
   KOHLMANN, Klaus
   VANDEPUTTE, Marc
   RODINA, Marek
   GELA, David
   LINHART, Otomar
AS 1:1;2:1;3:2;4:3,4;5:1;6:1;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Univ S Bohemia, Res Inst Fish Culture & Hydrobiol, Vodnany 38925, Czech Republic.
   Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Inland Fisheries, D-12561 Berlin, Germany.
   IFREMER, F-34250 Palavas Les Flots, France.
   INRA, UR544, Unite Genet Poissons, F-78350 Jouy En Josas, France.
C2 UNIV S BOHEMIA, CZECH REPUBLIC
   LEIBNIZ INST FRESHWATER ECOL & INLAND FISHERIES, GERMANY
   IFREMER, FRANCE
   INRA, FRANCE
SI PALAVAS
SE PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
IF 0.403
TC 9
UR https://archimer.ifremer.fr/doc/2008/publication-6551.pdf
LA English
DT Article
DE ;Paternity assignment;Artificial reproduction;Aquaculture
AB Sperm competition occurs when sperm from different males compete for fertilization. The aim of this in vitro fertilization study was to compare the competitive success of five males using heterosperm with an equal number of sperm from each male and four different sperm/egg ratios (5,000, 10,000, 20,000 and 100,000 spermatozoa per egg) in order to better understand variations of competitive fertilization. The roles of sperm motility and velocity were studied. Fertilization and hatching rates of the 5 males studied, with 100,000 spermatozoa per egg, were between 23.7-94.8% and 23.7-92.2%, respectively. Sperm velocity and percentage of motile sperm ranged from 85.0 to 137.6 mu m s(-1) and 2.0 to 93.5% at 15 see post sperm activation, respectively. The contribution of individual males to the progeny when using heterosperm was explored by DNA fingerprinting and was very diverse. Males with very low level of sperm motility (M3 4.45%, M4 1.95%) were represented by low contribution in all groups of progenies, but significant differences in the contribution of males were also found among individuals with a similar percentage of motile spermatozoa. The overall number of spermatozoa per egg strongly influenced the fertilization/hatching rate when using heterosperm, but had no impact on the relative number of progenies sired by each of the 5 competing males. Several models were tested in order to attempt to explain the contribution of individual males to the progeny.
PY 2008
PD JUN
SO Cybium, Revue Internationale d'Ichtyologie
SN 0399-0974
PU Société Française d'Ichtyologie
VL 32
IS 2
UT 000267276400149
BP 303
EP 306
ID 6551
ER

EF