FN Archimer Export Format PT J TI Biological characteristics of European hake (Merluccius merluccius) sperm BT AF GROISON, Anne-Laure SUQUET, Marc COSSON, Jacky LE COZ, Jean-Rene JOLIVET, Aurelie GARREN, Francois AS 1:1;2:2;3:3;4:4;5:5;6:5; FF 1:;2:PDG-DOP-DCB-PFOM-ARN;3:;4:;5:PDG-DOP-DCB-STH-LASAA;6:PDG-DOP-DCB-STH-LBH; C1 Univ Bergen, Dept Biol, N-5020 Bergen, Norway. IFREMER, PFOM, ARN, F-29840 Argenton, France. UPMC, CNRS, UMR 7009, Observ Oceanol, F-06230 Villefranche Sur Mer, France. IFREMER, PFOM PI, F-29280 Plouzane, France. IFREMER, STH LASAA, F-29280 Plouzane, France. C2 UNIV BERGEN, NORWAY IFREMER, FRANCE UNIV PARIS 06, FRANCE IFREMER, FRANCE IFREMER, FRANCE SI ARGENTON BREST SE PDG-DOP-DCB-PFOM-ARN PDG-DOP-DCB-STH-LASAA PDG-DOP-DCB-STH-LBH IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 0.403 TC 8 UR https://archimer.ifremer.fr/doc/2008/publication-6553.pdf LA English DT Article DE ;AEC;Gamete quality;Sperm motility;Sperm Density;Merluccius merluccius AB Very little is known on European hake's reproductive biology and especially on biological characteristics of its sperm despite a growing interest in its aquaculture potential. This study reports, for the first time some hake sperm characteristics. After activation, the swimming phase lasts 3 min (8 min when activated with 50% sea water (SW) but lower initial velocity). The initial flagellar beat frequency (BF), velocity and percentage of motile cells decreased after 100 s. After 2 days at 4 degrees C, the mean Adenylate Energy Charge (AEC) level was 0.71. Sperm stored at 4 degrees C still showed motility after 9 days for 2 individuals. When cryopreserved, the motility recovery index of thawed spermatozoa ranged from 11.8% to 29.6%. PY 2008 PD JUN SO Cybium, Revue Internationale d'Ichtyologie SN 0399-0974 PU Société Française d'Ichtyologie VL 32 IS 2 UT 000267276400076 ID 6553 ER EF