FN Archimer Export Format PT J TI Nautilia abyssi sp nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent BT AF ALAIN, Karine CALLAC, Nolwenn GUEGAN, Marianne LESONGEUR, Francoise CRASSOUS, Philippe CAMBON-BONAVITA, Marie-Anne QUERELLOU, Joel PRIEUR, Daniel AS 1:1;2:1;3:1;4:1;5:2;6:1;7:1;8:1; FF 1:;2:;3:;4:PDG-DOP-DCB-EEP-LMEE;5:PDG-DOP-DCB-EEP-LEP;6:PDG-DOP-DCB-EEP-LMEE;7:PDG-DOP-DCB-EEP-LMEE;8:; C1 IUEM, UMR6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France. IFREMER, EEP LEP, Lab Environm Profond, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-EEP-LMEE PDG-DOP-DCB-EEP-LEP IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.113 TC 20 UR https://archimer.ifremer.fr/doc/2009/publication-6585.pdf LA English DT Article AB A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209(T), was isolated from an East Pacific Rise hydrothermal vent (13 degrees N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 mu m) with a single polar flagellum. Strain PH1209(T) grew at temperatures between 33 and 65 degrees C (optimum 60 degrees C), from pH 5.0 to 8.0 (optimum 6.0-6.5), and between 2 and 4% (w/v) NaCl (optimum 3%). Cells grew chemolithoautotrophically with H-2 as an energy source, So as an electron acceptor and CO2 as a carbon source. Strain PH1209(T) was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic: analyses based on 16S rRNA gene sequencing showed that strain PH1209(T) fell within the order Nautiliales, in the class Epsilonproteobacteria. Comparative 16S rRNA gene sequence analysis indicated that strain PH1209(T) belonged to the genus Nautilia and shared 97.2 and 98.7% 16S rRNA gene sequence identity, respectively, with the type strains of Nautilia lithotrophica and Nautilia profundicola. It is proposed, from the polyphasic evidence, that the strain represents a novel species, Nautilia abyssi sp. nov.; the type strain is PH1209(T) (=DSM 21157(T)=JCM 15390(T)). PY 2009 PD JUL SO International journal of systematic and evolutionary microbiology SN 1466-5026 PU Society for General Microbiology VL 59 UT 000267645000010 BP 1310 EP 1315 DI 10.1099/ijs.0.005454-0 ID 6585 ER EF