FN Archimer Export Format
PT J
TI The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta
BT 
AF HENNEKE, Ghislaine
   RAFFIN, Jean-Paul
   FERRARI, Elena
   JONSSON, Zophonías
   DIETRICH, Jacques
   HUBSCHER, Ulrich
AS 1:;2:;3:;4:;5:;6:;
FF 1:PDG-DRV-VP-MBE;2:;3:;4:;5:PDG-DRV-RA-DRIM;6:;
C1 IFREMER, Lab Biotechnol Microorganismes Hydrothermaux, DRV, VP, F-29280 Plouzane, France.
   Univ Zurich, Inst Vet Biochem, CH-8057 Zurich, Switzerland.
C2 IFREMER, FRANCE
   UNIV ZURICH, SWITZERLAND
SI BREST
   MONTPELLIER
SE PDG-DRV-VP-MBE
   PDG-DRV-RA-DRIM
IN WOS Ifremer jusqu'en 2018
IF 3.055
TC 16
UR https://archimer.ifremer.fr/doc/2000/publication-659.pdf
LA English
DT Article
DE ;DNA polymerase delta;Conservation;Thermococcus fumicolans;Archaea;Hyperthermophile;PCNA
AB We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M-r of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals. 
PY 2000
PD SEP
SO Biochemical and Biophysical Research Communications
SN 0006-291X
PU Elsevier
VL 276
IS 2
UT 000089558600034
BP 600
EP 606
DI 10.1006/bbrc.2000.3481
ID 659
ER

EF