Phylogenetic characterization of the bacterial assemblage associated with mucous. secretions of the hydrothermal vent polychaete Paralvinella palmiformis
|Author(s)||Alain Karine, Olagnon Michel, Desbruyeres Daniel, Page Antoine, Barbier Georges, Juniper Kim, Querellou Joel, Cambon-Bonavita Marie-Anne|
|Affiliation(s)||IFREMER, Ctr Brest, Lab Microbiol & Biotechnol Extremophiles, Dept Valorisat Prod, F-29280 Plouzane, France.
IFREMER, Ctr Brest, Dept TMSI, F-29280 Plouzane, France.
IFREMER, Dept Environm Profond, Direct Rech Ocean, F-29280 Plouzane, France.
Univ Quebec, Geotop, Montreal, PQ H3C 3P8, Canada.
Univ Quebec, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada.
|Source||FEMS Microbiology Ecology (0168-6496) (Elsevier), 2002-12 , Vol. 42 , N. 3 , P. 463-476|
|WOS© Times Cited||74|
|Keyword(s)||Proteobacteria, 16S rRNA, Microbial diversity, Phylogeny, Hydrothermal vent, Paralvinella palmiformis|
|Abstract||As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. In both cases, clone libraries were largely dominated by epsilon-Proteobacteria. Phylotypes belonging to the Cytophaga-Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences were related to the taxonomic groups Fusobacteria, Green non-sulfur bacteria, Firmicutes, gamma- and delta-Proteobacteria. To our knowledge, this is the first report of the presence of Verrucomicrobia, Fusobacteria and green non-sulfur bacteria on hydrothermal edifices. The potential functions of the detected bacteria are discussed in terms of productivity, recycling of organic matter and detoxification within the P. palmiformis microhabitat.|