Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium

Type Article
Date 2003-10
Language English
Author(s) Alain Karine, Rolland Sophie, Crassous Philippe, Lesongeur Francoise, Zbinden Magali, Le Gall Christian, Godfroy AnneORCID, Page Antoine, Juniper S Kim, Cambon-Bonavita Marie-Anne, Duchiron Francis, Querellou Joel
Affiliation(s) IFREMER, Ctr Brest, Dept Valorisat Prod, Lab Microbiol & Biotechnol Extremophiles, F-29280 Plouzane, France.
Dept Environm Profond, Plouzane, France.
Lab Biol Cellulaire & Mol Dev, Paris, France.
Univ Quebec, Geotop, Montreal, PQ H3C 3P8, Canada.
Dept Sci Biol, Montreal, PQ, Canada.
Lab Microbiol Ind, Reims, France.
Source Extremophiles (1431-0651) (Springer), 2003-10 , Vol. 7 , N. 5 , P. 361-370
DOI 10.1007/s00792-003-0329-4
WOS© Times Cited 23
Keyword(s) Thermophile, Nitrate reducing, Lithotroph, Desulfurobacterium crinifex, Deep sea hydrothermal vent, Cell networks
Abstract A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200 kPa). Molecular hydrogen was the sole electron donor used by the strain. Nitrate and elemental sulfur served as electron acceptors, yielding ammonia and hydrogen sulfide, respectively (nitrate reduction supported higher growth rates than sulfur reduction). The G+C content of the genomic DNA was 36.7 +/- 0.8 mol%. Phylogenetic analyses of the 16S rRNA gene located the strain within the genus Desufurobacterium. However, the novel isolate possesses physiological and biochemical characteristics that differ from the previously described species of this genus. We propose that the isolate represents a novel species, Desulfurobacterium crinifex sp. nov. The type strain is NE1206(T) (DSM 15218(T), CIP 107649(T)). An amendment of the genus Desulfurobacterium description is proposed, based on the phenotypic characteristics of the novel species.
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Alain Karine, Rolland Sophie, Crassous Philippe, Lesongeur Francoise, Zbinden Magali, Le Gall Christian, Godfroy Anne, Page Antoine, Juniper S Kim, Cambon-Bonavita Marie-Anne, Duchiron Francis, Querellou Joel (2003). Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium. Extremophiles, 7(5), 361-370. Publisher's official version : https://doi.org/10.1007/s00792-003-0329-4 , Open Access version : https://archimer.ifremer.fr/doc/00000/663/