FN Archimer Export Format
PT J
TI In vivo RNA interference in oyster -vasa silencing inhibits germ cell development
BT 
AF FABIOUX, Caroline
   CORPOREAU, Charlotte
   QUILLIEN, Virgile
   FAVREL, Pascal
   HUVET, Arnaud
AS 1:1,2;2:2,3;3:2,3;4:2,3;5:2,3;
FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCB-PFOM-PI;3:PDG-DOP-DCB-PFOM-PI;4:;5:PDG-DOP-DCB-PFOM-PI;
C1 Univ Bretagne Occidentale, CNRS, UMR 6539, LEMAR,IUEM, Plouzane, France.
   Univ Caen, IFREMER, UMR 100, Ctr Brest,PEM2, F-14032 Caen, France.
   Univ Caen, IFREMER, UMR 100, IBFA,ICORE,PEM2,IFR 146, F-14032 Caen, France.
C2 UBO, FRANCE
   UNIV CAEN, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.042
TC 81
UR https://archimer.ifremer.fr/doc/2009/publication-6641.pdf
LA English
DT Article
DE ;Vasa;RNAi;Marine bivalve;Germline;Crassostrea gigas
AB This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies.
PY 2009
PD MAY
SO FEBS Journal
SN 1742-464X
PU Wiley / Blackwell
VL 276
IS 9
UT 000264882700013
BP 2566
EP 2573
DI 10.1111/j.1742-4658.2009.06982.x
ID 6641
ER

EF