FN Archimer Export Format PT J TI Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata) BT AF PANSERAT, Stéphane PLAGNES JUAN, E KAUSHIK, Sadasivam AS 1:;2:;3:; FF 1:;2:;3:; C1 INRA, IFREMER, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France. C2 INRA, FRANCE SI ST PEE SUR NIVELLE SE PDG-DRV-RA-ARN IN WOS Ifremer jusqu'en 2018 IF 1.713 TC 92 UR https://archimer.ifremer.fr/doc/2002/publication-668.pdf LA English DT Article DE ;Gilthead seabream;Common carp;Gluconeogenesis;Gene expression;Hepatic glucose metabolism;Dietary carbohydrate AB Our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. We compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata), known to be relatively tolerant to dietary carbohydrates. After cloning of partial cDNA sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (G6Pase), fructose biphosphatase (FBPase) and phosphoenolpyruvate carboxykinase (PEPCK) in the two species, we analyzed gene expressions of these enzymes 6 and 24 h after feeding with (20%) or without carbohydrates. Our data show that there is at least one gluconeogenic enzyme strongly regulated (decreased expression after feeding) in the two fish species, i.e. the PEPCK for common carp and G6Pase/FBPase for gilthead seabream. In these fish species, the regulation seems to be similar to the mammals at least at the molecular level. PY 2002 PD NOV SO Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression SN 0167-4781 PU Elsevier VL 1579 IS 1 UT 000178980800005 BP 35 EP 42 DI 10.1016/S0167-4781(02)00501-8 ID 668 ER EF