FN Archimer Export Format PT J TI Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio) BT AF PANSERAT, Stéphane BLIN, C MEDALE, Francoise PLAGNES JUAN, E BREQUE, Jeanine KRISHNAMOORTHY, J KAUSHIK, Sadasivam AS 1:;2:;3:;4:;5:;6:;7:; FF 1:;2:;3:;4:;5:;6:;7:; C1 INRA, IFREMER, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France. Hop Robert Debre, INSERM, U458, F-75019 Paris, France. C2 INRA, FRANCE HOP ROBERT DEBRE, FRANCE SI ST PEE SUR NIVELLE SE PDG-DRV-RA-ARN IN WOS Ifremer jusqu'en 2018 IF 1.849 TC 52 UR https://archimer.ifremer.fr/doc/2000/publication-669.pdf LA English DT Article DE ;Glucose phosphorylation;Dietary carbohydrate;Fish nutrition AB The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific expression (mainly liver). PY 2000 PD MAR SO Biochimica et Biophysica Acta (BBA) - General Subjects SN 0304-4165 PU Elsevier VL 1474 IS 1 UT 000085814100009 BP 61 EP 69 DI 10.1016/S0304-4165(99)00213-5 ID 669 ER EF