Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry

Type Article
Date 2004-07
Language English
Author(s) Petit Karina, Mondeguer FlorenceORCID, Roquebert M, Biard J, Pouchus Y
Affiliation(s) Univ Nantes, Fac Pharm, ISOMer, SMAB, F-44035 Nantes 01, France.
IFREMER, DEL, PN, F-44311 Nantes, France.
Museum Natl Hist Nat, Lab Cryptogamie, F-75005 Paris, France.
Source Journal of Microbiological Methods (0167-7012) (Elsevier), 2004-07 , Vol. 58 , N. 1 , P. 59-65
DOI 10.1016/j.mimet.2004.03.004
WOS© Times Cited 21
Keyword(s) Mass spectrometry ESI IT/MS, Griseofulvin, Antifungal, Marine fungi, Penicillium waksmanii
Abstract A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H](+). Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water.
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