Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain

Type Article
Date 2002-11
Language English
Author(s) Henneke Ghislaine, Gueguen YannickORCID, Flament Didier, Azam Philippe, Querellou Joel, Dietrich Jacques, Hubscher Ulrich, Raffin Jean-Paul, Henneke Ghislaine, Gueguen YannickORCID, Flament Didier, Azam Philippe, Querellou Joel, Dietrich Jacques, Hubscher Ulrich, Raffin Jean-Paul
Affiliation(s) IFREMER, Lab Microbiol & Biotechnol Extremophiles, DRV VP, F-29280 Plouzane, France.
Univ Zurich, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland.
Source Journal of Molecular Biology (0022-2836) (Elsevier), 2002-11 , Vol. 323 , N. 5 , P. 795-810
DOI 10.1016/S0022-2836(02)01028-8
WOS© Times Cited 24
Keyword(s) PCNA binding domain, Pyrococcus abyssi, Hyperthermophile, Archaea, Replication factor C
Abstract The molecular organization of the replication complex in archaea is similar to that in eukaryotes. Only two proteins homologous to subunits of eukaryotic replication factor C (RFC) have been detected in Pyrococcus abyssi (Pab). The genes encoding these two proteins are arranged in tandem. We cloned these two genes and co-expressed the corresponding recombinant proteins in Escherichia coli. Two inteins present in the gene encoding the small subunit (Pab RFC-small) were removed during cloning. The recombinant protein complex was purified by anion-exchange and hydroxyapatite chromatography. Also, the Pab RFC-small subunit could be purified, while the large subunit (Pab RFC-large) alone was completely insoluble. The highly purified Pab RFC complex possessed an ATPase activity, which was not enhanced by DNA. The Pab proliferating cell nuclear antigen (PCNA) activated the Pab RFC complex in a DNA-dependent manner, but the Pab RFC-small ATPase activity was neither DNA-dependent nor PCNA-dependent. The Pab RFC complex was able to stimulate Pab PCNA-dependent DNA synthesis by the Pab-family D heterodimeric DNA polymerase. Finally, (i) the Pab RFC-large fraction cross-reacted with anti-human-RFC PCNA-binding domain antibody, corroborating the conservation of the protein sequence, (ii) the human PCNA stimulated the Pab RFC complex ATPase activity in a DNA-dependent way and (iii) the Pab RFC complex could load human PCNA onto primed single-stranded circular DNA, suggesting that the PCNA-binding domain of RFC has been functionally conserved during evolution. In addition, ATP hydrolysis was not required either for DNA polymerase stimulation or PCNA-loading in vitro.
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Henneke Ghislaine, Gueguen Yannick, Flament Didier, Azam Philippe, Querellou Joel, Dietrich Jacques, Hubscher Ulrich, Raffin Jean-Paul, Henneke Ghislaine, Gueguen Yannick, Flament Didier, Azam Philippe, Querellou Joel, Dietrich Jacques, Hubscher Ulrich, Raffin Jean-Paul (2002). Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain. Journal of Molecular Biology, 323(5), 795-810. Publisher's official version : https://doi.org/10.1016/S0022-2836(02)01028-8 , Open Access version : https://archimer.ifremer.fr/doc/00000/675/