FN Archimer Export Format PT J TI Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae BT AF WOLD, P. -A. HOEHNE-REITAN, K. CAHU, Chantal ZAMBONINO-INFANTE, Jose-Luis RAINUZZO, J. KJORSVIK, E. AS 1:1;2:1;3:2;4:2;5:3;6:1; FF 1:;2:;3:PDG-DOP-DCB-PFOM;4:PDG-DOP-DCB-PFOM-ARN;5:;6:; C1 Norwegian Univ Sci & Technol, Dept Biol, N-7034 Trondheim, Norway. Inst Francais Rech Exploitat Mer, UMR Fish Nutr 1067, Plouzane, France. SINTEF Fisheries & Aquaculture, Trondheim, Norway. C2 UNIV SCI & TECHNOL NORWEGIAN, NORWAY IFREMER, FRANCE SINTEF FISHERIES & AQUACULTURE, NORWAY SI BREST SE PDG-DOP-DCB-PFOM PDG-DOP-DCB-PFOM-ARN IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.482 TC 29 UR https://archimer.ifremer.fr/doc/2009/publication-6777.pdf LA English DT Article DE ;Stereology;Phospholipids;Nutrition;Lipid class;Larvae;Histology;Diet;Cod AB The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly higher in NL1 larvae compared with the PL3 larvae. The liver and gut size was related to larval size, with no differences between the larval groups. The results demonstrated that the essential fatty acids were more beneficial for cod larvae when they were incorporated in the dietary polar PL rather than in the NL, and that the n-3 HUFA requirements in cod larvae is possibly higher than that in the PL1 diet. PY 2009 PD FEB SO Aquaculture Nutrition SN 1353-5773 PU Wiley / Blackwell VL 15 IS 1 UT 000262282000008 BP 73 EP 84 DI 10.1111/j.1365-2095.2008.00569.x ID 6777 ER EF