FN Archimer Export Format
PT J
TI Low-molecular-weight fucoidan enhances the proangiogenic phenotype of endothelial progenitor cells
BT 
AF ZEMANI, Faouzia
   BENISVY, Danielle
   GALY FAUROUX, Isabelle
   LOKAJCZYK, Anna
   COLLIEC-JOUAULT, Sylvia
   UZAN, Georges
   FISCHER, Anne Marie
   BOISSON VIDAL, Catherine
AS 1:1;2:1;3:1;4:1;5:2;6:3;7:1;8:1;
FF 1:;2:;3:;4:;5:PDG-DOP-DCB-BM-BMM;6:;7:;8:;
C1 INSERM, Fac Pharm, U428, F-75270 Paris, France.
   IFREMER, Nantes, France.
   Hop Paul Brousse, INSERM, U506, Villejuif, France.
C2 INSERM, FRANCE
   IFREMER, FRANCE
   HOP PAUL BROUSSE, FRANCE
SI NANTES
SE PDG-DOP-DCB-BM-BMM
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 3.617
TC 69
UR https://archimer.ifremer.fr/doc/2005/publication-679.pdf
LA English
DT Article
DE ;Fucoidan;FGF 2;Endothelial progenitor cells;Angiogenesis;Alpha 6
AB Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the presence of FGF-2 (p < 0.01). This latter effect correlated with upregulation of the cell-surface alpha 6 integrin subunit of the laminin receptor (P < 0.05). Compared to untreated HUVEC, untreated EPC alpha 6 expression and adhesion to laminin were enhanced two-fold. Fucoidan treatment further enhanced HUVEC but not EPC adhesion to laminin. These results show that fucoidan enhances the proangiogenic properties of EPC and suggest that ex vivo cc fucoidan preconditioning of EPC might lead to increased neovascularization when injected into ischemic tissues. 
PY 2005
PD OCT
SO Biochemical Pharmacology
SN 0006-2952
PU Elsevier
VL 70
IS 8
UT 000232391800006
BP 1167
EP 1175
DI 10.1016/j.bcp.2005.07.014
ID 679
ER

EF