FN Archimer Export Format PT J TI Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin BT AF ROUSSEL, Erwan SAUVADET, Anne-Laure CHADUTEAU, Carine FOUQUET, Yves CHARLOU, Jean-Luc PRIEUR, Daniel CAMBON, Marie-Anne AS 1:1;2:1;3:2;4:2;5:2;6:1;7:1; FF 1:PDG-DOP-DCB-EEP-LMEE;2:;3:PDG-DOP-DCB-GM-LGM;4:PDG-DOP-DCB-GM-LGM;5:PDG-DOP-DCB-GM-LGM;6:;7:PDG-DOP-DCB-EEP-LMEE; C1 Univ Bretagne Occidentale, CNRS, Inst Univ Europeen Mer, Lab Microbiol Environm Extremes,UMR 6197,Ifremer, F-29280 Plouzane, France. IFREMER, Ctr Brest, Dept Geosci Marines, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-EEP-LMEE PDG-DOP-DCB-GM-LGM IN WOS Ifremer jusqu'en 2018 copubli-univ-france IF 4.909 TC 38 TU Centre national de la recherche scientifique Institut français de recherche pour l'exploitation de la mer Université de Bretagne Occidentale UR https://archimer.ifremer.fr/doc/2009/publication-6801.pdf LA English DT Article CR MD 153 / AUSFAIR ZONECO12 BO Marion Dufresne DE ;marine subsurface sediments;sea floor biosphere;gradient gel electrophoresis;microbial communities;molecular diversity;anaerobic oxidation;sequence alignment;phylogenetic trees;extracellular DNA;methane hydrate AB P>The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured sedimentary Crenarchaeota. However, the anoxic subsurface methane-poor sediments (below 1.5 mbsf) were dominated by less active archaeal communities, such as the Thermoplasmatales, Marine Benthic Group D and other lineages probably involved in the methane cycle (Methanosarcinales, ANME-2 and DSAG/MBG-B). Moreover, the archaeal diversity of some sediment layers was restricted to only one lineage (Uncultured Euryarchaeota, DHVE6, MBG-B, MG-1 and SAGMEG). Sequences forming two clusters within the Thermococcales order were also present in these cold subseafloor sediments, suggesting that these uncultured putative thermophilic archaeal communities might have originated from a different environment. This study shows a transition between surface and subsurface sediment archaeal communities. PY 2009 PD SEP SO Environmental Microbiology SN 1462-2912 PU Wiley / Blackwell VL 11 IS 9 UT 000269539700024 BP 2446 EP 2462 DI 10.1111/j.1462-2920.2009.01976.x ID 6801 ER EF