FN Archimer Export Format
PT J
TI Structure and function of a novel endonuclease acting on branched DNA substrates
BT 
AF REN, Bin
   KUEHN, Joelle
   MESLET-CLADIERE, Laurence
   BRIFFOTAUX, Julien
   NORAIS, Cedric
   LAVIGNE, Regis
   FLAMENT, Didier
   LADENSTEIN, Rudolf
   MYLLYKALLIO, Hannu
AS 1:2;2:3;3:3;4:4,5;5:3;6:6;7:4,5;8:2;9:1,3;
FF 1:;2:;3:;4:PDG-DOP-DCB-EEP-LMEE;5:;6:;7:PDG-DOP-DCB-EEP-LMEE;8:;9:;
C1 Ecole Polytech, INSERM, Lab Opt & Biosci, CNRS,Unite Mixte Rech 7645,U696, F-91128 Palaiseau, France.
   Karolinska Inst, Novum, Ctr Struct Biochem, Huddinge, Sweden.
   Univ Paris 11, CNRS, Inst Genet & Microbiol, UMR8621, F-91405 Orsay, France.
   IFREMER, Lab Microbiol Environm Extremes, UMR 6197, Plouzane, France.
   Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, UMR 6197, Plouzane, France.
   Univ Rennes 1, INSERM, U625, Rennes, France.
C2 ECOLE POLYTECH, FRANCE
   KAROLINSKA INST, SWEDEN
   UNIV PARIS 11, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
   UNIV RENNES, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 8.993
TC 35
UR https://archimer.ifremer.fr/doc/2009/publication-6807.pdf
LA English
DT Article
DE ;Structure function studies;RecB family;Novel endonuclease;DNA repair;Branched DNA structures
AB We show that Pyrococcus abyssi PAB2263 (dubbed NucS (nuclease for ss DNA) is a novel archaeal endonuclease that interacts with the replication clamp PCNA. Structural determination of P. abyssi NucS revealed a two-domain dumbbell-like structure that in overall does not resemble any known protein structure. Biochemical and structural studies indicate that NucS orthologues use a non-catalytic ssDNA-binding domain to regulate the cleavage activity at another site, thus resulting into the specific cleavage at double-stranded DNA (dsDNA)/ssDNA junctions on branched DNA substrates. Both 3' and 5' extremities of the ssDNA can be cleaved at the nuclease channel that is too narrow to accommodate duplex DNA. Altogether, our data suggest that NucS proteins constitute a new family of structure-specific DNA endonucleases that are widely distributed in archaea and in bacteria, including Mycobacterium tuberculosis.
PY 2009
PD AUG
SO Embo Journal
SN 0261-4189
PU Nature Publishing Group
VL 28
IS 16
UT 000269074400016
BP 2479
EP 2489
DI 10.1038/emboj.2009.192
ID 6807
ER

EF