FN Archimer Export Format
PT J
TI Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy
BT 
AF LAURENT, Sebastien
   COLAS, Florent
   HAMELIN, Muriel
   CRASSOUS, Marie-Pierre
   ANTOINE, Elisabeth
   LEHAITRE, Michel
   COMPERE, Chantal
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;
FF 1:PDG-DOP-DCB-ERT-IC;2:PDG-DOP-DCB-ERT-IC;3:PDG-DOP-DCB-ERT-IC;4:PDG-DOP-DCB-ERT-IC;5:PDG-DOP-DCB-ERT-IC;6:PDG-DOP-DCB-ERT-IC;7:PDG-DOP-DCB-ERT-IC;
C1 IFREMER, ERT Interfaces & Capteurs, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
TC 1
UR https://archimer.ifremer.fr/doc/2009/publication-6830.pdf
LA English
DT Article
DE ;PCR;Algal detection;In situ;Spectroscopy;Surface plasmon resonance
AB Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
PY 2009
PD FEB
SO Sensors Applications Symposium, 2009. SAS 2009. IEEE
PU IEEE
UT 000268444000006
BP 29
EP 33
DI 10.1109/SAS.2009.4801771
ID 6830
ER

EF