FN Archimer Export Format PT J TI In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes BT AF GAGNAIRE, Beatrice THOMAS GUYON, H RENAULT, Tristan AS 1:;2:;3:; FF 1:PDG-DRV-RA-LGP;2:;3:PDG-DRV-RA-LGP; C1 IFREMER, LPG, F-17390 La Tremblade, France. Univ La Rochelle, LBEM, F-17042 La Rochelle, France. C2 IFREMER, FRANCE UNIV LA ROCHELLE, FRANCE SI LA TREMBLADE SE PDG-DRV-RA-LGP IN WOS Ifremer jusqu'en 2018 copubli-univ-france IF 2.149 TC 135 UR https://archimer.ifremer.fr/doc/2004/publication-691.pdf LA English DT Article DE ;Toxicity;Cellular activities;Phenoloxidase;Mercury;Cadmium;Flow cytometry;Haemocytes;Crassostrea gigas AB In the past decades. shellfish culture has developed in a significant way around the world. However, culture areas are Often Subject to recurring anthropic pollution. The recrudescent presence of industrial wastes is a source of heavy metals and results ill pollutant transfer towards the aquatic environment in estuarine areas. Because of their mode of life. bivalves. including Mussels and oysters. are suggested as ideal indicator organisms. The development of techniques allowing the analysis of the effects of Pollutants on bivalve biology may lead to the monitoring of pollutant transfer in estuarine areas. in this context. the effects of cadmium and mercury On defence mechanisms were analysed in Pacific oysters. Crassostrea gigas. Pollutant effects were tested in vitro oil oyster haemocytes. Cell viability and enzymatic activities (esterase. peroxidase. aminopeptidase, phagocytosis activities) were monitored by flow cytometry. Enzymatic phenoloxidase-like activity was also evaluated by spectrophotometry. High pollutant concentrations were used in order to detect the acute effect and to approach real Pollutant concentrations existing ill animal tissues. Cadmium induced no effect on oyster haemocytes under the tested conditions. On the contrary, mercury caused a significant haemocyte mortality after a 24 It in vitro incubation. Aminopeptidase positive cell percentage was enhanced by the pollutant, and phenoloxidase-like activity was inhibited. These in vitro results show that mercury may be expected to have tit impact oil bivalve immune functions in contaminated areas. PY 2004 PD APR SO Fish & Shellfish Immunology SN 1050-4648 PU Elsevier VL 16 IS 4 UT 000220922400004 BP 501 EP 512 DI 10.1016/j.fsi.2003.08.007 ID 691 ER EF