FN Archimer Export Format
PT J
TI Molecular detection and quantification of the protozoan Bonamia ostreae in the flat oyster, Ostrea edulis
BT 
AF ROBERT, Maeva
   GARCIA, Celine
   CHOLLET, Bruno
   LOPEZ-FLORES, Inmaculada
   FERRAND, Sylvie
   FRANCOIS, Cyrille
   JOLY, Jean-Pierre
   ARZUL, Isabelle
AS 1:1;2:1;3:1;4:2;5:1;6:1;7:1;8:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP;7:PDG-DOP-DCN-AGSAE-LGP;8:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Andalusian Sch Publ Hlth, CIBEResp, Granada 18080, Spain.
C2 IFREMER, FRANCE
   EASP, SPAIN
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.899
TC 19
UR https://archimer.ifremer.fr/doc/2009/publication-6934.pdf
LA English
DT Article
DE ;Heart imprints;Ostrea edulis;Quantification;Detection;Real time PCR;Bonamia ostreae
AB Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using infected oyster samples as templates, the assay was at least 10-fold more sensitive than conventional PCR. The quantitative assay was applied to test 132 oysters, and results were compared with the heart imprint method. There was a strong correlation between both techniques, and the results showed that the real-time PCR assay should be useful for studies of the ecology of B. ostreae and its host–parasite relationship.
PY 2009
PD DEC
SO Molecular and Cellular Probes
SN 0890-8508
PU Elsevier
VL 23
IS 6
UT 000272069100002
BP 264
EP 271
DI 10.1016/j.mcp.2009.06.002
ID 6934
ER

EF