|Author(s)||Luz Vilarino M.1, Le Guyader Soizick2, Polo David1, Schaeffer Julien2, Krol Joanna2, Romalde Jesus L.1|
|Affiliation(s)||1 : Univ Santiago de Compostela, Fac Biol, CIBUS, Dept Microbiol & Parasitol, Santiago De Compostela 15782, Spain.
2 : IFREMER, Microbiol Lab, Nantes, France.
|Source||International Microbiology (1139-6709) (Spanish Society for Microbiology (SEM) & Viguera Editores, S.L.), 2009-09 , Vol. 12 , N. 3 , P. 145-151|
|WOS© Times Cited||47|
|Keyword(s)||Seafood industry, Viral quantification, Viral prevalence, Norovirus, Hepatitis A virus, Enteric viruses, Molluscs|
|Abstract||Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 X 10(2) to 3.3 x 10(3) RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 x 10(1) to 1.5 x 10(4) RNA copies per gram of digestive tissue) than in wild (from 1.3 x 10(2) to 3.4 x 10(4) RNA copies per gram of digestive tissue) samples. [Int Microbioll 2009; 12(3):145-151]|
Luz Vilarino M., Le Guyader Soizick, Polo David, Schaeffer Julien, Krol Joanna, Romalde Jesus L. (2009). Assessment of human enteric viruses in cultured and wild bivalve molluscs. International Microbiology, 12(3), 145-151. Publisher's official version : https://doi.org/10.2436/20.1501.01.92 , Open Access version : https://archimer.ifremer.fr/doc/00000/6957/