Assessment of human enteric viruses in cultured and wild bivalve molluscs

Type Article
Date 2009-09
Language English
Author(s) Luz Vilarino M.1, Le Guyader Soizick2, Polo David1, Schaeffer Julien2, Krol Joanna2, Romalde Jesus L.1
Affiliation(s) 1 : Univ Santiago de Compostela, Fac Biol, CIBUS, Dept Microbiol & Parasitol, Santiago De Compostela 15782, Spain.
2 : IFREMER, Microbiol Lab, Nantes, France.
Source International Microbiology (1139-6709) (Spanish Society for Microbiology (SEM) & Viguera Editores, S.L.), 2009-09 , Vol. 12 , N. 3 , P. 145-151
DOI 10.2436/20.1501.01.92
WOS© Times Cited 44
Keyword(s) Seafood industry, Viral quantification, Viral prevalence, Norovirus, Hepatitis A virus, Enteric viruses, Molluscs
Abstract Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 X 10(2) to 3.3 x 10(3) RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 x 10(1) to 1.5 x 10(4) RNA copies per gram of digestive tissue) than in wild (from 1.3 x 10(2) to 3.4 x 10(4) RNA copies per gram of digestive tissue) samples. [Int Microbioll 2009; 12(3):145-151]
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