TY - JOUR T1 - Assessment of human enteric viruses in cultured and wild bivalve molluscs A1 - Luz Vilarino,M. A1 - Le Guyader,Soizick A1 - Polo,David A1 - Schaeffer,Julien A1 - Krol,Joanna A1 - Romalde,Jesus L. AD - Univ Santiago de Compostela, Fac Biol, CIBUS, Dept Microbiol & Parasitol, Santiago De Compostela 15782, Spain. AD - IFREMER, Microbiol Lab, Nantes, France. UR - https://archimer.ifremer.fr/doc/00000/6957/ DO - 10.2436/20.1501.01.92 KW - Seafood industry KW - Viral quantification KW - Viral prevalence KW - Norovirus KW - Hepatitis A virus KW - Enteric viruses KW - Molluscs N2 - Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 X 10(2) to 3.3 x 10(3) RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 x 10(1) to 1.5 x 10(4) RNA copies per gram of digestive tissue) than in wild (from 1.3 x 10(2) to 3.4 x 10(4) RNA copies per gram of digestive tissue) samples. [Int Microbioll 2009; 12(3):145-151] Y1 - 2009/09 PB - Spanish Society for Microbiology (SEM) & Viguera Editores, S.L. JF - International Microbiology SN - 1139-6709 VL - 12 IS - 3 SP - 145 EP - 151 ID - 6957 ER -