FN Archimer Export Format PT J TI Assessment of human enteric viruses in cultured and wild bivalve molluscs BT AF LUZ VILARINO, M. LE GUYADER, Soizick POLO, David SCHAEFFER, Julien KROL, Joanna ROMALDE, Jesus L. AS 1:1;2:2;3:1;4:2;5:2;6:1; FF 1:;2:PDG-DOP-DCN-EMP-MIC;3:;4:PDG-DOP-DCN-EMP-MIC;5:PDG-DOP-DCN-EMP-MIC;6:; C1 Univ Santiago de Compostela, Fac Biol, CIBUS, Dept Microbiol & Parasitol, Santiago De Compostela 15782, Spain. IFREMER, Microbiol Lab, Nantes, France. C2 UNIV SANTIAGO DE COMPOSTELA, SPAIN IFREMER, FRANCE SI NANTES SE PDG-DOP-DCN-EMP-MIC IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.8 TC 48 UR https://archimer.ifremer.fr/doc/2009/publication-6957.pdf LA English DT Article DE ;Seafood industry;Viral quantification;Viral prevalence;Norovirus;Hepatitis A virus;Enteric viruses;Molluscs AB Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 X 10(2) to 3.3 x 10(3) RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 x 10(1) to 1.5 x 10(4) RNA copies per gram of digestive tissue) than in wild (from 1.3 x 10(2) to 3.4 x 10(4) RNA copies per gram of digestive tissue) samples. [Int Microbioll 2009; 12(3):145-151] PY 2009 PD SEP SO International Microbiology SN 1139-6709 PU Spanish Society for Microbiology (SEM) & Viguera Editores, S.L. VL 12 IS 3 UT 000270132100001 BP 145 EP 151 DI 10.2436/20.1501.01.92 ID 6957 ER EF