FN Archimer Export Format PT J TI Lack of significant long-term effect of dietary carbohydrates on hepatic glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss) BT AF PANSERAT, Stéphane MEDALE, Francoise BREQUE, Jeanine PLAGNES JUAN, E KAUSHIK, Sadasivam AS 1:;2:;3:;4:;5:; FF 1:;2:;3:;4:;5:; C1 IFREMER, INRA, Lab Fish Nutr, F-64310 St Pee Sur Nivelle, France. C2 IFREMER, FRANCE SI ST PEE SUR NIVELLE SE PDG-DRV-RA-ARN IN WOS Ifremer jusqu'en 2018 IF 1.083 TC 115 UR https://archimer.ifremer.fr/doc/2000/publication-696.pdf LA English DT Article DE ;Dietary carbohydrates;Rainbow trout;Fish nutrition;Glucose 6 phosphatase expression AB Hepatic glucose-6-phosphatase (G6Pase) plays an important role in glucose metabolism because it catalyzes the release of glucose to the circulatory system in the processes of glycogenolysis and gluconeogenesis. The present study was initiated to analyze the regulation of hepatic G6Pase expression by dietary carbohydrates in rainbow trout. The first step in our study was the identification of a partial G6Pase cDNA in rainbow trout that was highly homologous to that of mammals. Hepatic G6Pase activities and mRNA levels were measured in trout fed one of the experimental diets, with or without carbohydrates. We found no significant effect of intake of dietary carbohydrates on G6Pase Expression (mRNA and activity) 6 hours and 24 hours after feeding. These results suggest that there is no control of G6Pase synthesis by dietary carbohydrates in rainbow trout and that the lack of regulation of gluconeogenesis by dietary carbohydrates could at least partially explain the postprandial hyperglycemia and the low dietary glucose utilization observed in this species. (J. Nutr. Biochem. 11:22-29, 2000) PY 2000 SO The Journal of Nutritional Biochemistry SN 0955-2863 PU Elsevier VL 11 IS 1 UT 000085397100003 BP 22 EP 29 DI 10.1016/S0955-2863(99)00067-4 ID 696 ER EF