Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses

Type Article
Date 2002-04
Language English
Author(s) Mari Jocelyne, Poulos Bonnie, Lightner Donald, Bonami Jean-Robert
Affiliation(s) Univ Arizona, Dept Vet Sci & Microbiol, Aquaculture Pathol Grp, Tucson, AZ 85721 USA.
CNRS, IFREMER, UM2, UMR 5098,CC080, F-34095 Montpellier 5, France.
Source Journal of General Virology (0022-1317) (Society for General Microbiology), 2002-04 , Vol. 83 , P. 915-926
WOS© Times Cited 114
Keyword(s) Genomic characterization, Taura syndrome, Virus, Shrimp disease, Penaeidae
Abstract The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of apoptosis proteins from double-stranded DNA viruses and from animals. The presence of this BIR-like sequence is the first reported in a single-stranded RNA virus, but its function is unknown. The N-terminal amino acid sequence of three TSV capsid proteins (55, 40 and 24 kDa) were mapped in ORF2, which is not in the same reading frame as ORF1 and possesses an AUG codon upstream of the structural genes. However, the intergenic region shows nucleotide sequence similarity with those of the genus Cricket paralysis-like viruses, suggesting a similar non-AUG-mediated translation mechanism. The structure of the TSV genome [5' UTR-non-structural proteins-intergenic UTR-structural proteins-3' UTR-poly(A) tail] is similar to those of small insect-infecting RNA viruses, which were recently regrouped into a new virus genus, Cricket paralysis-like viruses.
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Mari Jocelyne, Poulos Bonnie, Lightner Donald, Bonami Jean-Robert (2002). Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses. Journal of General Virology, 83, 915-926. Open Access version : https://archimer.ifremer.fr/doc/00000/715/