Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin

Type Article
Date 2009-11
Language English
Author(s) Cambon-Bonavita Marie-AnneORCID1, Nadalig Thierry1, 2, Roussel ErwanORCID1, Delage Eloise1, Duperron Sebastien1, 2, Caprais Jean-Claude2, Boetius A.3, Sibuet Myriam2
Affiliation(s) 1 : IFREMER, Brest DEEP LM2E, UMR 6197, F-29280 Plouzane, France.
2 : IFREMER, Brest DEEP LEP, F-29280 Plouzane, France.
3 : Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany.
Source Deep Sea Research Part II: Topical Studies in Oceanography (0967-0645) (Elsevier), 2009-11 , Vol. 56 , N. 23 , P. 2248-2258
DOI 10.1016/j.dsr2.2009.04.007
WOS© Times Cited 35
Keyword(s) Microbial phylogeny, Faunal assemblage, Cold seep, Chemosynthetic ecosystems, AOM, REGAB
Abstract A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups were detected in the pockmark sediments, as well as from both known bacterial partners. The archaeal diversity was limited to the ANME cluster for all investigated samples. The bacterial diversity included members of the Proteobacteria, Bacilliales, Cytophaga/Flavobacteria, Verrucomicrobia, JS1 and Actinobacteria clusters. Bacterial 16S rRNA gene sequences related to those of known sulphide-oxidizing symbionts were recovered from tissues of several invertebrates including vesicomyid clams and siboglinid tubeworms of REGAB.
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Cambon-Bonavita Marie-Anne, Nadalig Thierry, Roussel Erwan, Delage Eloise, Duperron Sebastien, Caprais Jean-Claude, Boetius A., Sibuet Myriam (2009). Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin. Deep Sea Research Part II: Topical Studies in Oceanography, 56(23), 2248-2258. Publisher's official version : https://doi.org/10.1016/j.dsr2.2009.04.007 , Open Access version : https://archimer.ifremer.fr/doc/00000/7298/