FN Archimer Export Format PT J TI Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin BT AF CAMBON-BONAVITA, Marie-Anne NADALIG, Thierry ROUSSEL, Erwan DELAGE, Eloise DUPERRON, Sebastien CAPRAIS, Jean-Claude BOETIUS, A. SIBUET, Myriam AS 1:1;2:1,2;3:1;4:1;5:1,2;6:2;7:3;8:2; FF 1:PDG-DOP-DCB-EEP-LMEE;2:PDG-DOP-DCB-EEP-LEP;3:PDG-DOP-DCB-EEP-LMEE;4:PDG-DOP-DCB-EEP-LMEE;5:PDG-DOP-DCB-EEP-LEP;6:PDG-DOP-DCB-EEP-LEP;7:;8:; C1 IFREMER, Brest DEEP LM2E, UMR 6197, F-29280 Plouzane, France. IFREMER, Brest DEEP LEP, F-29280 Plouzane, France. Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany. C2 IFREMER, FRANCE IFREMER, FRANCE MAX PLANCK INST, GERMANY SI BREST SE PDG-DOP-DCB-EEP-LMEE PDG-DOP-DCB-EEP-LEP IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.965 TC 37 TU Centre national de la recherche scientifique Institut français de recherche pour l'exploitation de la mer Université de Bretagne Occidentale UR https://archimer.ifremer.fr/doc/2009/publication-7298.pdf LA English DT Article CR BIOZAIRE 1 BIOZAIRE 2 ZAIANGO-BIOL2 ZAIANGO1 ZAIANGO2 BO Unknown Anchored Buoy Thalassa L'Atalante DE ;Microbial phylogeny;Faunal assemblage;Cold seep;Chemosynthetic ecosystems;AOM;REGAB AB A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups were detected in the pockmark sediments, as well as from both known bacterial partners. The archaeal diversity was limited to the ANME cluster for all investigated samples. The bacterial diversity included members of the Proteobacteria, Bacilliales, Cytophaga/Flavobacteria, Verrucomicrobia, JS1 and Actinobacteria clusters. Bacterial 16S rRNA gene sequences related to those of known sulphide-oxidizing symbionts were recovered from tissues of several invertebrates including vesicomyid clams and siboglinid tubeworms of REGAB. PY 2009 PD NOV SO Deep Sea Research Part II: Topical Studies in Oceanography SN 0967-0645 PU Elsevier VL 56 IS 23 UT 000272366000009 BP 2248 EP 2258 DI 10.1016/j.dsr2.2009.04.007 ID 7298 ER EF