FN Archimer Export Format
PT J
TI Binding to PCNA in Euryarchaeal DNA Replication Requires Two PIP Motifs for DNA Polymerase D and One PIP Motif for DNA Polymerase B
BT 
AF CASTREC, Benoit
   ROUILLON, Christophe
   HENNEKE, Ghislaine
   FLAMENT, Didier
   QUERELLOU, Joel
   RAFFIN, Jean-Paul
AS 1:1,2;2:1,2;3:1,2;4:1,2;5:1,2;6:2,3;
FF 1:;2:PDG-DOP-DCB-EEP-LMEE;3:PDG-DOP-DCB-EEP-LMEE;4:PDG-DOP-DCB-EEP-LMEE;5:PDG-DOP-DCB-EEP-LMEE;6:;
C1 Univ Bretagne Occidentale, UMR 6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
   IFREMER, UMR 6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
   CNRS, UMR 6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.871
TC 24
UR https://archimer.ifremer.fr/doc/2009/publication-7317.pdf
LA English
DT Article
DE ;Archaea;PIP box;PCNA binding motifs;DNA polymerases;DNA replication
AB Replicative DNA polymerases possess a canonical C-terminal proliferating cell nuclear antigen (PCNA)-binding motif termed the PCNA-interacting protein (PIP) box. We investigated the role of the PIP box on the functional interactions of the two DNA polymerases, PabPol B (family B) and PabPol D (family D), from the hyperthermophilic euryarchaeon Pyrococcus abyssi, with its cognate PCNA. The PIP box was essential for interactions of PabPol B with PCNA, as shown by surface plasmon resonance and primer extension studies. In contrast, binding of PabPol D to PCNA was affected only partially by removing the PIP motif. We identified a second palindromic PIP box motif at the N-terminus of the large subunit of PabPol D that was required for the interactions of PabPol D with PCNA. Thus, two PIP motifs were needed for PabPol D for binding to PabPCNA. Moreover, the C-terminus of PabPCNA was essential for stimulation of PabPol D activity but not for stimulation of PabPol B activity. Neither DNA polymerase interacted with the PabPCNA interdomain connecting loop. Our data suggest that distinct processes are involved in PabPol D and PabPol B binding to PCNA, raising the possibility that Archaea require two mechanisms for recruiting replicative DNA polymerases at the replication fork. (C) 2009 Elsevier Ltd. All rights reserved.
PY 2009
PD NOV
SO Journal of Molecular Biology
SN 0022-2836
PU Elsevier
VL 394
IS 2
UT 000271985700004
BP 209
EP 218
DI 10.1016/j.jmb.2009.09.044
ID 7317
ER

EF