FN Archimer Export Format
PT J
TI Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005-2008 survey
BT 
AF LI, Hui
   TOUBIANA, Mylene
   MONFORT, Patrick
   ROCH, Philippe
AS 1:1;2:1;3:1;4:1;
FF 1:;2:;3:;4:;
C1 Univ Montpellier 2, CNRS, IFREMER, JRU, F-34095 Montpellier 05, France.
C2 UNIV MONTPELLIER, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-DRV-RA-DRIM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.29
TC 22
UR https://archimer.ifremer.fr/doc/2009/publication-7345.pdf
LA English
DT Article
DE ;Mollusks;Mytilus;Innate immunity;Seasonal variations;Immune survey;Q PCR;HSP70;Lysozyme;Myticin;Mytilin;Defensin;Antimicrobial peptide
AB Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring-summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on 28S rRNA, defensin, myticin 11 and lysozyme expressions, but not on mytilin B and HSP70. The positive influence was significant for defensin and lysozyme expression, but relationships cannot be quantified. Similarly, salinity appeared to influence defensin expression, but this relationship cannot be quantified neither. E coli tissue content appeared without influence. Consequently, there was no clear relationship between environmental parameters and immune-related gene expressions, demonstrating anti-infectious capabilities cannot be evaluated using only the expression of such genes as markers. (C) 2009 Elsevier Ltd. All rights reserved.
PY 2009
PD SEP
SO Developmental & Comparative Immunology
SN 0145-305X
PU Elsevier
VL 33
IS 9
UT 000268088800004
BP 974
EP 979
DI 10.1016/j.dci.2009.04.002
ID 7345
ER

EF