FN Archimer Export Format
PT J
TI Influence of environmental parameters on Karenia selliformis toxin content in culture
BT 
AF MEDHIOUB, Amel
   MEDHIOUB, Walid
   AMZIL, Zouher
   SIBAT, Manoella
   BARDOUIL, Michele
   BEN NEILA, Idriss
   MEZGHANI, Salah
   HAMZA, Asma
   LASSUS, Patrick
AS 1:1;2:2;3:2;4:2;5:2;6:3;7:3;8:1;9:2;
FF 1:;2:;3:PDG-DOP-DCN-EMP-PHYC;4:PDG-DOP-DCN-EMP-PHYC;5:PDG-DOP-DCN-EMP-PHYC;6:;7:;8:;9:PDG-DOP-DCN-EMP-PHYC;
C1 INSTM, Lab Aquaculture, Salammbo 2025, Tunisia.
   IFREMER, Dept Environm,Microbiol & Phycotoxines, F-44311 Nantes, France.
   Ctr Reg Sfax, IRVT, Sfax 3003, Tunisia.
C2 INSTM, TUNISIA
   IFREMER, FRANCE
   IRVT, TUNISIA
SI NANTES
SE PDG-DOP-DCN-EMP-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 0.873
TC 16
UR https://archimer.ifremer.fr/doc/2009/publication-7383.pdf
LA English
DT Article
DE ;Mouse bioassay;Gymnodimine;Toxicity;Growth;Karenia selliformis
AB Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabes gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae oil enriched Gaillard f/2 medium. The influence of growing conditions oil toxin content was studied, examining the effects of (i) different culture Volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15 degrees C et 20-21 degrees C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 +/- 0.14 div d(-1)) was obtained at 20 degrees C and a salinity of 36, (ii) GYM content expressed as pg eq GYM cell(-1) increased with Culture time. The neurotoxicity of K. selliformis extracts was confirmed by mouse bioassay. This study allowed us to calculate the minimal lethal dose (MLD) of gymnodimine (GYM) that kills a mouse, as a function of the number of K. selliformis cells extracted.
PY 2009
PD DEC
SO CBM - Cahiers de Biologie Marine
SN 0007-9723
PU Station Biologique de Roscoff
VL 50
IS 4
UT 000273205200005
BP 333
EP 342
ID 7383
ER

EF