TY - JOUR T1 - Vibrio gigantis sp nov., isolated from the haemolymph of cultured oysters (Crassostrea gigas) A1 - Le Roux,Frederique A1 - Goubet,A A1 - Thompson,F A1 - Faury,Nicole A1 - Gay,Melanie A1 - Swings,J A1 - Saulnier,Denis AD - IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. AD - State Univ Ghent, Lab Microbiol & BCCM, LMG Bacteria Collect, Microbiol Lab, B-9000 Ghent, Belgium. UR - https://archimer.ifremer.fr/doc/00000/792/ DO - 10.1099/ijs.0.63666-0 KW - Vibrio gigantis KW - Haemoplymph KW - Disease KW - Crassostrea gigas KW - Oyster culture N2 - Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase Quo factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and beta-galactosidase activities, NO2 production, growth at 35 degrees C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13(T) (=LMG 22741(T)=CIP 108656(T)). Y1 - 2005/06 PB - Society for General Microbiology JF - International journal of systematic and evolutionary microbiology SN - 1466-5026 VL - 55 IS - 6 SP - 2251 EP - 2255 ID - 792 ER -