FN Archimer Export Format PT J TI Sea bass Dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity to sea bass larvae BT AF BREUIL, Gilles MOUCHEL, Olivier FAUVEL, Christian PEPIN, Jean-Francois AS 1:;2:;3:;4:; FF 1:PDG-DRV-RA-LPALAVAS;2:PDG-DRV-VP-BPQ;3:PDG-DRV-RA-LPALAVAS;4:PDG-DRV-RA-LPALAVAS; C1 IFREMER, Lab Rech Aquacole, F-34250 Palavas Les Flots, France. C2 IFREMER, FRANCE SI PALAVAS NANTES SE PDG-DRV-RA-LPALAVAS PDG-DRV-VP-BPQ IN WOS Ifremer jusqu'en 2018 IF 1.653 TC 43 UR https://archimer.ifremer.fr/doc/2001/publication-814.pdf LA English DT Article DE ;ELISA;Nodavirus;Fish encephalopathy AB Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2 experimentally infected larvae, as well as in naturally infected sea bass larvae collected in French hatcheries or in barramundi larvae reared in the Pacific area. The development of an ELISA specific for the 2 nodavirus strains isolated from the sea bass should be useful for the detection of the virus, in addition to other techniques recommended by the Office International des Epizooties (OIE). PY 2001 PD MAY SO Diseases of aquatic organisms SN 0177-5103 PU Inter-Research VL 45 IS 1 UT 000168965300004 BP 25 EP 31 ID 814 ER EF