FN Archimer Export Format
PT J
TI Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast
BT 
AF RENAULT, Tristan
   STOKES, Nancy
   CHOLLET, Bruno
   COCHENNEC, Nathalie
   BERTHE, Franck
   GERARD, Andre
   BURRESON, Eugene
AS 1:;2:;3:;4:;5:;6:;7:;
FF 1:PDG-DRV-RA-LGP;2:;3:PDG-DRV-RA-LGP;4:PDG-DRV-RA-DCOP-AT;5:PDG-DRV-RA-LGP;6:PDG-DRV-RA;7:;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Coll William & Mary, Virginia Inst Marine Sci, Gloucester Point, VA 23062 USA.
C2 IFREMER, FRANCE
   COLL WILLIAM & MARY, USA
SI LA TREMBLADE
   TAHITI
   NANTES
SE PDG-DRV-RA-LGP
   PDG-DRV-RA-DCOP-AT
   PDG-DRV-RA
IN WOS Ifremer jusqu'en 2018
IF 1.556
TC 65
UR https://archimer.ifremer.fr/doc/2000/publication-821.pdf
LA English
DT Article
DE ;Haplosporidium nelsoni;Haplosporidiosis;Crassostrea gigas;Pacific oyster
AB Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A series of primers was designed to amplify short regions of small subunit ribosomal DNA (SSU rDNA) from most haplosporidians. The primers encompass a highly variable region of the SSU rDNA and did not amplify oyster DNA. PCR amplification of the infected C, gigas genomic DNA with these primers yielded the expected-sized product from the primer pair targeting the shortest region (94 bp). This PCR product was sequenced and it was identical to the corresponding SSU rDNA region of H. nelsoni.
PY 2000
PD SEP
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 42
IS 3
UT 000165643500006
BP 207
EP 214
ID 821
ER

EF