FN Archimer Export Format PT J TI Digestive enzyme activity and food ingesta in juvenile shrimp Litopenaeus vannamei (Boone, 1931) as a function of body weight BT AF GAMBOA DELGADO, Julián MOLINA POVEDA, César CAHU, Chantal AS 1:;2:;3:; FF 1:;2:;3:PDG-DRV-RA-ARN; C1 Fdn CENAIM ESPOL, Guayaquil, Ecuador. IFREMER, Ctr Brest, INRA, Unite Mixte Nutr Poisson, Plouzane, France. C2 FDN CENAIM ESPOL, ECUADOR IFREMER, FRANCE SI BREST SE PDG-DRV-RA-ARN IN WOS Ifremer jusqu'en 2018 IF 0.766 TC 60 UR https://archimer.ifremer.fr/doc/2003/publication-902.pdf LA English DT Article DE ;Semi intensive culture;Stomach content;Digestive enzymes;Litopenaeus vannamei;Shrimp AB A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp, respectively, decreasing to 33-43% at greater shrimp weights. Artificial feed showed a maximum contribution of 20% in 6-g shrimp. The present results show changes in the enzyme activity after the shrimp reach 6 g in body weight, evidenced by a decrease in total protease and an increase in lipase and amylase activities. The amylase/protease ratio was 2.6 in 2-g shrimp and increased steadily to 9.6 in 12-g shrimp. These findings suggest an adaptation of the enzymatic activity to diets with lower protein content as body weight increases, and may be related to the variation of the different items found in the stomach. PY 2003 PD DEC SO Aquaculture Research SN 1355-557X PU Blackwell science VL 34 IS 15 UT 000187154600005 BP 1403 EP 1411 DI 10.1111/j.1365-2109.2003.00959.x ID 902 ER EF